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Abstract.
Plant
propagation by tissue culture is known as micropropagation. Four
sequential stages are known in micropropagation system are as under:
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Establishment
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Multiplication
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Pretransplant
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Transplant
The basic procedure for stage
one is to establish the explant in culture, screen for contaminated
explant and allow excised plant unit to stabilized to the culture
environment. Any plant tissue or organ could be used as explant
source, but it success depend upon the culture system, species being
cultured, and removal of the contaminant from the explants .The stage
one provide large percentage of explants free from surface pathogens.
In establishment stage a sterile explant is produced and planted on
sterile basal medium, which include, semisolid medium, agar, mineral
salts with minor and major element, sucrose use as a source of energy,
vitamin supplements, hormone, plant growth regulator, cytokinin, auzin
and gibberellin. Usually 4-6 weeks required to complete stage one. |
